In vitro and in silico assessment of the effect of WWOX expression on invasiveness pathways associated with AP-2 transcription factors in bladder cancer

Table 3 Real-time qPCR primer sequences, amplification conditions and product length for target genes

Gene	Primer sequence	Product [bp]	Annealing [°C]	Reading [°C]
ACVR1B	^5′CTGACCCTTCCATTGAGGAAAT^3′ ^5′CCGCAGTGCCTCATAACTC^3′	95	60	60
CRABP2	^5′CCTGTAAGAGCCTGGTGAAA^3′ ^5′ATCGTTGGTCAGTTCTCTGG^3′	110	60	60
CDKN1A	^5′GACAGACAACTCACTCGTCAAA^3′ ^5′CATGGCGCCTGAACAGAA^3′	86	60	60
TP63	^5′GAAACCAGAGATGGGCAAGT^3′ ^5′ATGCTATCTTCATCCGCCTTC^3′	95	60	60
AP1M2	^5′AAGCCACTGATCTGGATTGAG^3′ ^5′CCTTGGCCTTGACCATGA^3′	76	60	60
IKBKB	^5′CTGTATCCTTCAAGAGCCCAAG^3′ ^5′CCGGTTGCAATCTTCCTTCA^3′	106	60	60
SMAD4	^5′ATGGATACGTGGACCCTTCT^3′ ^5′ATGTGCAACCTTGCTCTCTC^3′	97	60	60

ISSN: 1471-2490