In vitro and in silico assessment of the effect of WWOX expression on invasiveness pathways associated with AP-2 transcription factors in bladder cancer

Table 2 Real-time qPCR primer sequences, amplification conditions and product length for reference genes

Gene	Primer sequence	Product [bp]	Annealing [°C]	Reading [°C]
H3F3A	^5′ACGGATTACACCTTCCCACTTGCTAAAAGGTC^3′ ^5′AGCCACAAAGGCAGATGGATCAGCCAAG^3′	69	65	72
RPLP0	^5′AGGACTTTAAAAGATCTGCGCTTCCAGAG^3′ ^5′ACCAGATAGGCCTCACTTGCCTCCTGC^3′	76	65	72
RPS17	^5′AAGCGCGTGTGCGAGGAGATCG^3′ ^5′TCGCTTCATCAGATGCGTGACATAACCTG^3′	87	64	72

ISSN: 1471-2490